What are the limitations of using pharmacological activators of AMPK?

The AMP activated protein kinase (AMPK) enzyme complex (consisting of 3 distinct subunits) is major energy sensor whose role is to maintain cellular energy ... The AMPK Kinase Assay uses the Transcreener ADP² Assay to directly detect ADP produced by AMPK to measure enzymatic activity. Unique direct ADP detection method ... ... (AMPK/mTOR)-mediated autophagy and upregulation of host antioxidant ... Enzyme Expression and Enhancing Viral Replication in GF-1 Cells. Viruses, 16 ... Jan 1, 2018 ... ... Enzyme Activation, Enzyme Activators, Enzyme Inhibitors, Fibroblasts, Fluorescent Antibody Technique, Humans, Immunoblotting, Macrolides ... Dec 16, 2017 ... ATIC suppresses AMPK activation, thus activates mTOR-S6 K1-S6 signaling and supports growth and motility activity of HCC cells. Conclusion. Nov 18, 2019 ... The impaired gene expression of adenosine monophosphate-activated kinase (AMPK), a key metabolic enzyme in leukocytes of newly diagnosed ... Aug 21, 2024 ... AMP-activated protein kinase (AMPK) is the master regulator of cellular and organismal energy homeostasis, playing an essential role in ... ... AMPK enzyme, while phosphorylation ot Ser24/25 and Ser182 affects AMPK localization (7). Accumulating evidence indicates that AMPK not only regulates the ... The ability to inhibit AMPK activity allows researchers to explore the enzyme's role in various metabolic and cellular processes, including glucose ... Dec 18, 2023 ... Here, we identified diacylglycerol lipase-beta (DAGLβ) as a principal regulator of AMP-activated protein kinase (AMPK) activation in primary ...