what is non-homologous end joining (NHEJ) and why is it considered more error-prone compared to template-based repair

DNA polymerase, an enzyme, recognizes each base in a template strand and matches it to the complementary base in a free nucleotide. The enzyme then catalyzes ... Oct 4, 2007 ... ... polymerase on cell activity with intervening on the DNA template itself. Polymerase is critically causally important, but varying its ... ... template for replication like the structure of DNA. DNA and proteins are thus complementary in function: DNA supplies the information needed to determine ... 2 U Taq DNA polymerase/30 µL PCR buffer with 400–500 µM dNTPs and 2.5. µM ... with 100 ng of template DNA. It follows from the presented data that 27 ... ... DNA polymer by the pattern in which the four ... One of the DNA strands in the double helix holds the genetic information used for protein synthesis. ... DNA (cDNA) using mRNA as the template. This yields a ... The Court appears to have concluded that isolated DNA is naturally occurring in the informational ... ... DNA recognition site of the archaeal homing endonuclease I-DmoI. JO ... informational databases, has been AB - performed and corresponding diagrams of ... Templates are single-stranded DNA or RNA polymers containing genetic information that directs the synthesis of new DNA, RNA or protein polymers during ... Abstract. Family A DNA polymerases (PolAs) form an important and well-studied class of extant polymerases participating in DNA replication and repair. None. For a DNA plasmid, this may include sterility, endotoxin, purity. (including percent of supercoiled form and residual cell DNA, RNA, and protein levels), and ...