


how does the field of synthetic biology utilize our understanding of dna replication and template-directed synthesis to create novel biological systems or molecules
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what is a template in dna replication Informational
- how do post-translational modifications (e.g., phosphorylation, ubiquitination) regulate the activity and interactions of replication proteins at the template
- how are interstrand crosslinks in the dna template resolved during replication
- what is the role of DNA ligase in joining Okazaki fragments synthesized using the lagging strand template
- how do errors that occur during template replication contribute to genetic diversity and evolution over long periods
- how does mismatch repair further enhance the accuracy of replication by identifying and correcting base-pairing errors that escape the polymerase proofreading, based on the template strand identity
- what is nucleotide excision repair (NER) and how does it remove bulky lesions that distort the DNA template
- how does the cell cycle machinery regulate the assembly and disassembly of these replication factories and the recruitment of the dna template to them
- what are some of the diseases associated with defects in mitochondrial dna replication and template integrity
- what enzymes interact with the template strand during dna replication
- how can understanding the vulnerabilities of dna replication, particularly at the template level, be exploited for cancer therapy
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