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what is retention factor
- How is the intermediate precision or ruggedness of a method assessed with respect to retention time and factor?
- What is the significance of retention factor in capillary electrophoresis?
- How are changes to a validated method managed?
- How does the percentage of organic modifier in the mobile phase affect retention in RPLC?
- What are the key parameters that need to be optimized during method development to ensure adequate separation and sensitivity?
- What are the limitations of using retention factor?
- What is the Gibbs free energy change associated with retention?
- What are the challenges in separating and identifying closely related compounds with similar retention factors?
- What are the advantages of affinity chromatography for target molecule isolation?
- How can knowledge of analyte properties help predict retention factor?
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