What are the techniques for studying organelles?

... mammalian cells exposed to ionizing radiation. The RFA will emphasize direct analysis of the genes and gene products associated with ionizing irradiation of ... Description. The 9th international conference on Systems Biology of Mammalian Cells SBMC 2024 will take place on 13 – 15 May in Leipzig, Germany. More ... We have wild type and several glyco-engineered HEK293s strains growing in serum free suspension to produce different glycoforms of therapeutic proteins. Stow, J. L. and Teasdale, R. D. (2005). Expression and localization of proteins in mammalian cells. Encyclopedia of Genetics, Genomics, Proteomics and ... abstract. To overproduce biologically active polyomavirus middle T antigen, we used an adenovirus vector and human 293 cells as hosts. The in vitro micronucleus test is a genotoxicity test for the detection of micronuclei in the cytoplasm of interphase cells. Micronuclei may originate from ... We developed a combinatorial RNA interference (RNAi) based platform for the systematic and quantitative generation of genetic and chemical-genetic interaction ... By analyzing the localization of Anap-labeled proteins in various mammalian cells, we demonstrate that the fluorescence of Anap is bright enough to be useful as ... This article describes how mammalian cells are derived, their utility, and the processes that harness their full potential. A complete serum-free medium developed for robust cell growth and protein expression for a wide range of mammalian cells including 293, CHO and hybridoma lines.