What are the challenges in working with primary mammalian cells?

Mar 25, 2014 ... Plasmid transfection into mammalian cells is fairly straightforward and the resultant cells can either express the plasmid DNA transiently ( ... This article describes how mammalian cells are derived, their utility, and the processes that harness their full potential. These results demonstrate that gene expression in mammalian cells is subject to large, intrinsically random fluctuations and raise questions about how cells ... We developed a combinatorial RNA interference (RNAi) based platform for the systematic and quantitative generation of genetic and chemical-genetic interaction ... abstract. To overproduce biologically active polyomavirus middle T antigen, we used an adenovirus vector and human 293 cells as hosts. 1), appears to also induce cell cycle arrest in mammalian cell lines. The ... M4N causes growth arrest in mammalian cells. C3, TC-1, C33a, and CEM-T4 ... A complete serum-free medium developed for robust cell growth and protein expression for a wide range of mammalian cells including 293, CHO and hybridoma lines. Dec 31, 2024 ... ... mammalian cells. We expressed these sarabodies and sarabody–GFP modules, purified them as soluble proteins, characterized their binding ... Starvation induces diffusion hindrance at the nanoscale in mammalian cells. S. Sareen, A. Zgorzelska, K. Kwapiszewska and R. Hołyst, Nanoscale, 2025, 17, 378 ... Our Gibco, Thermo Scientific Nunc, and Invitrogen cell culture products help you to achieve reproducible results and successful research outcomes—every day ...