What are the challenges in delivering CRISPR-Cas9 to specific cell types?

Cell-cell transfer of mRNA and its role in mammalian cell physiology. mRNA in tunneling nanotube. HER2 mRNA (red) visualized by single-molecule FISH in ... Dec 31, 2024 ... ... mammalian cells. We expressed these sarabodies and sarabody–GFP modules, purified them as soluble proteins, characterized their binding ... High frequency retrotransposition in cultured mammalian cells. Cell. 1996 Nov 29;87(5):917-27. doi: 10.1016/s0092-8674(00)81998-4. abstract. To overproduce biologically active polyomavirus middle T antigen, we used an adenovirus vector and human 293 cells as hosts. Mammalian Cell Lysis Buffer 5X ... Mammalian Cell Lysis Buffer 5X (ab179835) is widely used to prepare mammalian cell and tissue lysates for use in a variety of ... By analyzing the localization of Anap-labeled proteins in various mammalian cells, we demonstrate that the fluorescence of Anap is bright enough to be useful as ... An efficient DNA damage response is critical for maintaining the integrity of the mammalian genome, and ensuring the accurate transfer of genetic ... Cells isolated from animal tissues can be expanded in culture to study cell biology and disease or used for the production of antibodies, proteins, and vaccines ... Abstract. DNA-calcium phosphate co-precipitates arise spontaneously in supersaturated solutions. Highly effective precipitates for transfection purposes, ... We developed a combinatorial RNA interference (RNAi) based platform for the systematic and quantitative generation of genetic and chemical-genetic interaction ...