What are the applications of CRISPR-Cas9 in mammalian cell research?

We have wild type and several glyco-engineered HEK293s strains growing in serum free suspension to produce different glycoforms of therapeutic proteins. Sep 27, 2019 ... Mammalian acoustic reporter genes allow cells to be visualized at volumetric densities below 0.5% and permit high-resolution imaging of gene expression in ... A complete serum-free medium developed for robust cell growth and protein expression for a wide range of mammalian cells including 293, CHO and hybridoma lines. Yes, it is possible to isolate plasmid DNA from mammalian cells using the QIAprep Spin Miniprep kit. Cell-cell transfer of mRNA and its role in mammalian cell physiology. mRNA in tunneling nanotube. HER2 mRNA (red) visualized by single-molecule FISH in ... Mammalian cells are gaining popularity in expressing protein as there is more flexibility in pursuing this route. However, unlike plant cells or yeast, ... To ensure genome stability, mammalian cells employ several DNA repair pathways. Nonhomologous DNA end joining (NHEJ) is the DNA repair process that fixes ... The in vitro mammalian cell gene mutation test can be used to detect gene mutations induced by chemical substances. In this test, the used genetic endpoints ... Sep 27, 2022 ... Here, we report progress on a large-scale synthetic genomics effort to reestablish EAA biosynthetic functionality in mammalian cells. We offer complete solutions for cell line and process development, late phase activities, clinical and commercial manufacturing for a wide variety of ...