What are the alternative gene editing technologies?

To ensure genome stability, mammalian cells employ several DNA repair pathways. Nonhomologous DNA end joining (NHEJ) is the DNA repair process that fixes ... We have wild type and several glyco-engineered HEK293s strains growing in serum free suspension to produce different glycoforms of therapeutic proteins. ... mammalian cells exposed to ionizing radiation. The RFA will emphasize direct analysis of the genes and gene products associated with ionizing irradiation of ... High frequency retrotransposition in cultured mammalian cells. Cell. 1996 Nov 29;87(5):917-27. doi: 10.1016/s0092-8674(00)81998-4. Here, we systematically analyzed the biodiversity of R2 elements and screened several R2 orthologs capable of full-length gene insertion in mammalian cells. Sep 27, 2022 ... Here, we report progress on a large-scale synthetic genomics effort to reestablish EAA biosynthetic functionality in mammalian cells. 1), appears to also induce cell cycle arrest in mammalian cell lines. The ... M4N causes growth arrest in mammalian cells. C3, TC-1, C33a, and CEM-T4 ... abstract. To overproduce biologically active polyomavirus middle T antigen, we used an adenovirus vector and human 293 cells as hosts. Jun 27, 2011 ... ... mammalian muscle cells (2). We propose that cell chiral alignment is associated with cell migration based on the following observations: (i) ... May 17, 2024 ... Here, we measured transport and metabolism of individual lipid species in mammalian cells using time-resolved fluorescence imaging of bifunctional lipid probes.